In vivo thrombus formation is enhanced in Syk(Y317F) KI mice with increased annexin V binding compared with WT. (A) Scatter dot plot shows the time it took for bleeding to stop during tail bleeding experiments conducted on Syk(Y317F) and WT littermate control mice 4 to 6 weeks of age in a blind fashion. (B) Scatter dot plot of the time to occlusion in WT and Syk(Y317F) mice following 7.5% FeCl₃ injury on the carotid artery. Normality of the data was assessed using Shapiro-Wilk test, and data were found to be normally distributed. Accordingly, P values were determined using unpaired Student t test. (C-D) Bar graphs represent MFI and percentage of annexin V–positive platelets with different doses of CRP stimulation, respectively. Results are depicted as mean ± SEM, and differences are analyzed using 2-way ANOVA with Tukey post hoc test. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. MFI, median fluorescence intensities.