Figure 2.
Enhanced GPVI-mediated aggregation and secretion in Syk(Y317F) platelets. (A) Representative aggregation and secretion tracings of Syk(Y317F) and WT control platelets after stimulation with the indicated concentrations of CRP. (B) Expression of GPVI on CD41+ cells is shown as a flow histogram. Staining of Syk(Y317F) and WT platelets with fluorescein isothiocyanate (FITC)–conjugated anti-GPVI is compared with isotype control FITC-labeled immunoglobulin G1. (C) Quantification of aggregation (left) and ATP secretion (right). (D) Quantification of P-selectin (left) and JON/A (right) expression. Results are depicted as mean ± standard error of the mean (SEM), and differences are analyzed using 2-way analysis of variance (ANOVA) with Tukey post hoc test. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.

Enhanced GPVI-mediated aggregation and secretion in Syk(Y317F) platelets. (A) Representative aggregation and secretion tracings of Syk(Y317F) and WT control platelets after stimulation with the indicated concentrations of CRP. (B) Expression of GPVI on CD41+ cells is shown as a flow histogram. Staining of Syk(Y317F) and WT platelets with fluorescein isothiocyanate (FITC)–conjugated anti-GPVI is compared with isotype control FITC-labeled immunoglobulin G1. (C) Quantification of aggregation (left) and ATP secretion (right). (D) Quantification of P-selectin (left) and JON/A (right) expression. Results are depicted as mean ± standard error of the mean (SEM), and differences are analyzed using 2-way analysis of variance (ANOVA) with Tukey post hoc test. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.

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