Anti-ADAMTS13 antibodies display high affinity for ADAMTS13. (A-H) Binding between 7 patient-derived anti-CUB antibodies and 1 antispacer antibody (1-416), and wtADAMTS13 as determined by SPR (n = 2). Figures display association (first 360 seconds) and dissociation (rest of the chromatogram) curves upon flowing of various concentration of ADAMTS13 across a chip coated with the respective antibody, the binding that occurs is expressed in response units. (I) Table of binding constants as determined by fitting of the experimental data using a 1:1 binding model. Green, 30 nM ADAMTS13; purple, 10 nM ADAMTS13; gold, 3.33 nM ADAMTS13; light blue, 1.11 nM ADAMTS13; and magenta, 0.37 nM ADAMTS13. Black, fit according to 1:1 binding model.
Figure 1.

Anti-ADAMTS13 antibodies display high affinity for ADAMTS13. (A-H) Binding between 7 patient-derived anti-CUB antibodies and 1 antispacer antibody (1-416), and wtADAMTS13 as determined by SPR (n = 2). Figures display association (first 360 seconds) and dissociation (rest of the chromatogram) curves upon flowing of various concentration of ADAMTS13 across a chip coated with the respective antibody, the binding that occurs is expressed in response units. (I) Table of binding constants as determined by fitting of the experimental data using a 1:1 binding model. Green, 30 nM ADAMTS13; purple, 10 nM ADAMTS13; gold, 3.33 nM ADAMTS13; light blue, 1.11 nM ADAMTS13; and magenta, 0.37 nM ADAMTS13. Black, fit according to 1:1 binding model.

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