CpG dinucleotides subject to ATC-induced C>G transversions are highly methylated. (A) Methylation status of C>G mutated CpG for Ptpn11. C1-C3 represent individual plasmid subclones from polymerase chain reaction amplification of bisulfite treated RAG-1 KO leukemic DNA (top), and C1-C4 represent subclones from bisulfite-treated RAG-1 KO nonleukemic BCP DNA (bottom). Individual CpG are numbered 1-9. Dotted red boxes indicate CpG at known sites of C>G transversion in this study. Dark gray circles indicate methylated cytosines, white circles indicate unmethylated cytosines, and blue circles indicate C>G transversions. (B) Methylation status of C>G mutated CpG dinucleotides at Cdh23. (C) Methylation status of C>A mutation at CpG dinucleotides within Jak1. Green circle indicates a C>A mutation known to be present. (D) Methylation status of C>T mutation at CpG dinucleotide within Ebf1. Orange circles indicate a C>T mutation known to be present. (E) Methylation status of C>G transversion at non-CpG dinucleotides within Gata1. G in red represents C>G mutation at a non-CpG dinucleotide which was known to be present in this sample based on WES. “T” in blue represents bisulfite conversion of nonmethylated C.