BCP-ALL developing after ATC exposure shows a dramatic increase in C>G transversions. (A) Absolute number of C>G and non-C>G transversions identified for RAG-1 KO ATC B-lineage leukemia samples (n = 8) using WES. (B) Absolute number of C>A (left plot) and C>T (right plot) SBS mutations compared to remaining SBS mutations (excluding C>G transversions) identified from WES data. ∗∗∗P = .0002. (C) Representative SBS plot of RAG-1 KO BCP-ALL from mouse 8660. Note most C>G transversions are in a 5'-NCG-3' context. (D) SBS plot of RAG-1 KO BCP-ALL from mouse 8660 following computational removal of C>G mutations and re-scaling mutation count; this allows visualization of non-C>G mutations. C>A and C>T mutations are also in a 5'-NCG-3' context.