Gene expression analysis highlights enrichment of both murine and human BCP-ALL signatures in RAG-1 KO ATC CD19⁺ B220⁺ B-lineage ALL. (A) Principal component analysis plot generated from DeSeq2 normalized RNA sequencing data. Samples include 6 RAG-1 KO ATC B-lineage leukemias, 3 untreated RAG-1 KO CD19⁺B220⁺ sorted BM cells and 2 RAG-1 KO B220⁺ sorted BM cells. (B) Hierarchical clustering for selected genes associated with B-cell development. (C) GSEA comparing a murine BCP-ALL gene set (GSE221597) and differentially expressed genes from RAG-1 KO BCP-ALL samples (n = 6) vs CD19⁺B220⁺ sorted BM cells (n = 3). (D) GSEA comparing human BCP-ALL gene set (GSE79533) and differentially expressed genes from RAG-1 KO BCP-ALL vs CD19⁺B220⁺ sorted BM. FDR, false discover rate; NES, normalized enrichment score.