A stable method to purify and recover human ILC2 cells ex vivo. Human ILC2 cells were enriched from the same apheresis unit cells using either the RosetteSep human ILC2 enrichment kit, EasySep human ILC2 enrichment kit, or a combination of EasySep human NK cell enrichment kit and EasySep human CD56 positive selection II kit. Data are representative of 3 separate experiments. (A) Flow cytometric profiles of pre-enrichment and postenrichment samples divided by lineage markers (Lin) and CD127. (B) Lin^–CD127⁺ gated populations divided by CD161 and CRTH2. (C) Graph showing the yield of ILC2 cells using the 3 different enrichment methods. Blood samples were from 3 donors split into test samples, 1 for each kit. Each symbol represents an individual donor; small horizontal lines indicate the group mean (± standard deviation). (D) A graph showing the absolute cell numbers of human ILC2 cells enriched from an apheresis unit of PB cells using RosetteSep human ILC2 enrichment kit, 6 different donors; small horizontal line indicates the mean (± standard deviation).