MO induces hypoxia stress and promotes erythropoiesis in E13.5 and E17.5 embryonic liver. (A) Experimental workflow for scRNA-seq of the E13.5 and E17.5 fetal livers from CT and MO groups. (B) Dot plot showing specific cell marker genes for each cluster. (C) UMAP projection displayed major hepatic and hematopoietic cell types. (D) Bar plot comparing cell type proportions between CT and MO groups. (E) GO enrichment analysis of upregulated genes in MO group, highlighting erythroid development and oxidative stress-related processes. (F-H) GSEA shows positive enrichment of oxygen-related response pathways in MO fetal liver cells. The scRNA-seq was performed using pooled embryos (n = 3 pregnancies per group; 3 embryos per pregnancy) for both E13.5 and E17.5 time points. UMAP, uniform manifold approximation and projection.
Figure 2.

MO induces hypoxia stress and promotes erythropoiesis in E13.5 and E17.5 embryonic liver. (A) Experimental workflow for scRNA-seq of the E13.5 and E17.5 fetal livers from CT and MO groups. (B) Dot plot showing specific cell marker genes for each cluster. (C) UMAP projection displayed major hepatic and hematopoietic cell types. (D) Bar plot comparing cell type proportions between CT and MO groups. (E) GO enrichment analysis of upregulated genes in MO group, highlighting erythroid development and oxidative stress-related processes. (F-H) GSEA shows positive enrichment of oxygen-related response pathways in MO fetal liver cells. The scRNA-seq was performed using pooled embryos (n = 3 pregnancies per group; 3 embryos per pregnancy) for both E13.5 and E17.5 time points. UMAP, uniform manifold approximation and projection.

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