Highly enriched inflammatory gene expression signatures in Vk^∗MYC:Wwox KO PBTs. (A) Unsupervised clustering and heat map of 2355 differentially expressed genes (FDR < 0.05) comparing Vk^∗MYC:Wwox KO-BM (n = 3) and Vk^∗MYC:Wwox KO-PBT (n = 4) groups. Red or blue colors indicate differentially upregulated or downregulated genes, respectively. Mean signals were background corrected and log₂ transformed. (B-C) Bar graph depicting enrichment of biofunctions associated with tumorigenic phenotypes (B) and disease processes (C) (highlighted by red arrows), driven by dysregulated gene expression in Vk^∗MYC:Wwox KO PBTs (z score cutoff ±2; P < .05). (D) Bar graph showing the number of deregulated genes and the magnitude of statistical significance for enriched biofunctions and disease processes in Vk^∗MYC:Wwox KO-PBT vs Vk^∗MYC:Wwox KO-BM and Vk^∗MYC:Wwox KO-BM vs Vk^∗MYC:Wwox WT-BM groups. The enrichment was more pronounced in Vk^∗MYC Wwox KO-PBT vs Vk^∗MYC Wwox KO-BM (P value range, 4.28×10^-40 to 1.69×10^-19) compared to Vk^∗MYC:Wwox KO-BM vs Vk^∗MYC:Wwox WT-BM (P value range, 2.06×10^-7 to 1.19E×10^-3). Numbers in parentheses beside each bar indicate the number of differentially expressed genes in each category.