Fas mediates FasL-induced caspase activation and apoptosis in human T and myeloid cells in vitro.(A) Fas protein level on human T lymphoma and myeloid leukemia cell surface. The cells were stained with human Fas-specific antibody and analyzed by flow cytometry. (B) The 4 cell lines as shown in panel A were treated with recombinant FasL at the indicated concentrations in vitro for ∼24 hours. Cells were then stained with annexin V and propidium iodide (PI) and analyzed for annexin V+ and PI+ cells. Shown are representative dot plots. (C) The annexin V+PI+ cells as shown in panel B were gated and quantified as apoptotic cell death. (D) The 4 cell lines were treated with FasL (10 ng/mL) in vitro and analyzed by western blotting for caspase 3 activation and PARP cleavage. IgG, immunoglobulin G; PARP, poly (ADP-ribose) polymerase.
Figure 1.

Fas mediates FasL-induced caspase activation and apoptosis in human T and myeloid cells in vitro.(A) Fas protein level on human T lymphoma and myeloid leukemia cell surface. The cells were stained with human Fas-specific antibody and analyzed by flow cytometry. (B) The 4 cell lines as shown in panel A were treated with recombinant FasL at the indicated concentrations in vitro for ∼24 hours. Cells were then stained with annexin V and propidium iodide (PI) and analyzed for annexin V+ and PI+ cells. Shown are representative dot plots. (C) The annexin V+PI+ cells as shown in panel B were gated and quantified as apoptotic cell death. (D) The 4 cell lines were treated with FasL (10 ng/mL) in vitro and analyzed by western blotting for caspase 3 activation and PARP cleavage. IgG, immunoglobulin G; PARP, poly (ADP-ribose) polymerase.

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