Antibiotic suppression of peripheral lymphocytes occurs rapidly with the depletion of the intestinal microbiota. (A) Peripheral blood lymphocyte counts were assessed on an automated hematologic cell counter in Ctrl- and Abx-treated WT mice over 14 days. (B) Fecal pellets revealed a decreased relative abundance of fecal microbes in Abx-treated WT mice over 14 days compared with Ctrl mice as determined by 16S rRNA gene qPCR. (C) Mass of spleens isolated from mice given Abx for the indicated number of days. (D) Flow cytometric determination of the absolute number of B220+ B cells from the spleens of mice from panel C. For panels A-B, 2-way mixed analysis of variance was used to test statistical significance between groups of interest; and for panels C-D, the Kruskal-Wallis test was used. Data are displayed as mean ± SEM from 2 independent experiments. ns, P > .05; ∗P ≤ .05; ∗∗P ≤ .01; ∗∗∗P ≤ .001; ∗∗∗∗P ≤ .0001. ns, not significant.
Figure 2.

Antibiotic suppression of peripheral lymphocytes occurs rapidly with the depletion of the intestinal microbiota. (A) Peripheral blood lymphocyte counts were assessed on an automated hematologic cell counter in Ctrl- and Abx-treated WT mice over 14 days. (B) Fecal pellets revealed a decreased relative abundance of fecal microbes in Abx-treated WT mice over 14 days compared with Ctrl mice as determined by 16S rRNA gene qPCR. (C) Mass of spleens isolated from mice given Abx for the indicated number of days. (D) Flow cytometric determination of the absolute number of B220+ B cells from the spleens of mice from panel C. For panels A-B, 2-way mixed analysis of variance was used to test statistical significance between groups of interest; and for panels C-D, the Kruskal-Wallis test was used. Data are displayed as mean ± SEM from 2 independent experiments. ns, P > .05; ∗P ≤ .05; ∗∗P ≤ .01; ∗∗∗P ≤ .001; ∗∗∗∗P ≤ .0001. ns, not significant.

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