Effects of PAX5::ELN oncoprotein expression in the absence of IL7R. (A) Immunophenotypic characterization of medullar B-cell populations. UMAP (Uniform Manifold Approximation and Projection) of the clustering analysis of the B-cell subpopulations from 3-week- and 3-month-old mice with the indicated genotypes. Eight surface markers were targeted to identify the subpopulations: B220, CD19, CD117, CD43, IL7R, BP1, CD25, and IgM (supplemental Figure 1 and supplemental Materials and Methods). (B) Percentages of the medullar B-cell subpopulations, from 3-week- and 3-month-old mice with the indicated genotypes. Results are expressed as mean ± SD (GraphPad Prism) and overall differences between values were evaluated by t test with Mann–Whitney U posttest. The difference between means is significant if ∗P value <.05, very significant if ∗∗P value <.01, extremely significant if ∗∗∗P value <.001, or if ∗∗∗∗P value <.0001. (C) Kaplan-Meier survival curves. The genotypes and the numbers of mice are indicated. The follow-up was stopped in the 15th month. Throughout the study, the mutant mice were hemizygous for the human PAX5::ELN–encoding cDNA insertion and homozygous for IL7R-deficiency. IgM, immunoglobulin M; SD, standard deviation; WT, wild type.
Figure 1.

Effects of PAX5::ELN oncoprotein expression in the absence of IL7R. (A) Immunophenotypic characterization of medullar B-cell populations. UMAP (Uniform Manifold Approximation and Projection) of the clustering analysis of the B-cell subpopulations from 3-week- and 3-month-old mice with the indicated genotypes. Eight surface markers were targeted to identify the subpopulations: B220, CD19, CD117, CD43, IL7R, BP1, CD25, and IgM (supplemental Figure 1 and supplemental Materials and Methods). (B) Percentages of the medullar B-cell subpopulations, from 3-week- and 3-month-old mice with the indicated genotypes. Results are expressed as mean ± SD (GraphPad Prism) and overall differences between values were evaluated by t test with Mann–Whitney U posttest. The difference between means is significant if ∗P value <.05, very significant if ∗∗P value <.01, extremely significant if ∗∗∗P value <.001, or if ∗∗∗∗P value <.0001. (C) Kaplan-Meier survival curves. The genotypes and the numbers of mice are indicated. The follow-up was stopped in the 15th month. Throughout the study, the mutant mice were hemizygous for the human PAX5::ELN–encoding cDNA insertion and homozygous for IL7R-deficiency. IgM, immunoglobulin M; SD, standard deviation; WT, wild type.

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