Xenograft experiment to test the OR-2100 plus CHOP regimen. (A) Experimental schema for the RC cell xenograft mouse model. BRJ double-deficient mice were inoculated subcutaneously with 5 × 10⁶ RC cells and then treated with vehicle (n = 10), OR-2100 (3.39 mg/kg; n = 9), as indicated by the red arrowheads. (B) The mean tumor volume was measured twice per week, starting on day 1. Ctr, gray; OR-2100, purple. (C) The mean tumor weight in each treatment group. Differences between the vehicle and each treatment group were evaluated using Dunnett test. (D) Image of the resected tumors in each treatment group. (E) Mean body weight of mice. No body weight loss was noted in any of the groups Ctr, gray; OR-2100, purple. (F) The concentration of Hb and complete blood counts, including WBC and PLT counts, in mice euthanized at 14 days after the first treatment. (G) Experimental schema for the RC cell xenograft mouse model. BRJ double-deficient mice were inoculated subcutaneously with 5 × 10⁶ RC cells and then treated with vehicle (n = 5), OR-2100 (3.39 mg/kg; n = 6), CHOP (n = 5), or OR-2100 + CHOP (n = 6), as indicated by the red and blue arrowheads. (H) The mean tumor volume was measured twice per week, starting on day 1. Ctr, gray; OR-2100: purple; CHOP: blue; OR-2100 + CHOP, lavender. (I) The mean tumor weight in each treatment group. Differences between the vehicle and each treatment group were tested using Dunnett’s test. ns, not significant. (J) The mean body weight of mice. No body weight loss was noted in any of the 4 groups. Ctr, gray; OR-2100, purple; CHOP, blue; OR-2100 + CHOP, lavender. (K) The concentration of Hb and complete blood counts, including WBC and PLT counts, in mice sacrificed at 22 days after the first treatment. The differences between the CHOP and OR-2100 + CHOP group were tested using Dunnett test. Hb, hemoglobin; ns, not significant; PLT, platelet; WBC, white blood cell.