Evaluation of the TP53-dependent mechanism of OR-2100. (A) RC and OCI-Ly18 cells were transduced with shRNA that targeted TP53, and the reduction in TP53 expression was confirmed by immunoblotting. (B) The cells were treated with OR-2100 for 72 hours, followed by assessment of the cell viability. (C) The protein expression levels of β-tubulin and borealin (CDCA8) in RC and OCI-Ly18 cells treated with OR-2100 was assessed by western blotting. The representative images demonstrate that the protein levels of borealin was more profoundly downregulated in shCtr cells than in shTP53 cells.
Figure 5.

Evaluation of the TP53-dependent mechanism of OR-2100. (A) RC and OCI-Ly18 cells were transduced with shRNA that targeted TP53, and the reduction in TP53 expression was confirmed by immunoblotting. (B) The cells were treated with OR-2100 for 72 hours, followed by assessment of the cell viability. (C) The protein expression levels of β-tubulin and borealin (CDCA8) in RC and OCI-Ly18 cells treated with OR-2100 was assessed by western blotting. The representative images demonstrate that the protein levels of borealin was more profoundly downregulated in shCtr cells than in shTP53 cells.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal