Functional analysis of CDCA8 or BIRC5 in DHL cell lines in vitro and in vivo. (A) RC cells and OCI-Ly18 cells were transduced with shRNA targeting CDCA8 and BIRC5, respectively. A reduction in the expression of borealin (CDCA8) and survivin (BIRC5) was confirmed by immunoblotting. (B) Cell counts for the shCtr cells and shCDCA8 cells. Red lines indicate the cells infected with lentiviral vectors that lacked CDCA8. Gray lines indicate the cells infected with empty vectors. (C) Cell counts for the shCtr cells and shBIRC5 cells. Blue lines indicate the cells infected with lentiviral vectors lacking BIRC5. Gray lines indicate the cells infected with empty vectors. (D) A comparison of the cell counts after knockdown of both CDCA8 and BIRC5 with Ctr (gray, Ctr; purple, shCDCA8 and BIRC5). (E) Experimental schema for the OCI-Ly18 cell xenograft mouse model used to evaluate CDCA8 knockdown. Volume (F) or weight (G) of xenografts formed by OCI-Ly18 cells infected with empty vector (shCtr; gray line) or shCDCA8 (red line). cont, control.