YX0798 induces enrichment of OXPHOS and excessive oxidative stress in MCL cells. (A) Enriched signaling pathways upon treatment with YX0798 and AZD4573 for 6 hours. (B) Illustration of enrichment of oxidative phosphorylation and RESPIRATORY_ELECTRON_TRANSPORT pathways leading to excessive oxidative stress and ROS production. (C) Enrichment of REACTOME_ROS_AND_RNS_PRODUCTION_IN_PHAGOCYTES signaling pathway upon treatment with YX0798 and AZD4573 for 6 hours. (D) ROS production is elevated upon treatment with YX0798 and AZD4573 for 6 and 24 hours in Mino and JeKo-1 cells. Flow cytometry results of cellular ROS levels in cells (left). Quantitative results of cellular ROS levels in cells (right). (E) NAC pretreatment for 16 hours promoted cell survival and protected JeKo-1 cells from apoptosis induced by YX0798 treatment for 5 hours. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. NAC, N-acetyl cysteine; ns, not significant; ssGSEA, single-sample GSEA.