Erythroid-specific GPX4 KO mice have higher lipid peroxidation and lower PTR. RBCs from EpoR-Cre GPX4f/f mice and control (GPX4f/f) mice were stored for 7 days under refrigerated conditions (n = 3), prior to PTR studies, which showed a significant drop in PTR in conditional GPX4 KO mice (A). After confirming GPX4 ablation through proteomics (B), we identified the top 50 metabolites (C) and proteins (D) affected by genotype and storage time (time series analysis of variance). Highlights include decreased consumption of free reduced glutathione (E), and increased accumulation of oxidized glutathione (F), increases in lipid hydroperoxides, and depletion of glutathionylated-adducts of lipid hydroperoxides (G-H).
Figure 1.

Erythroid-specific GPX4 KO mice have higher lipid peroxidation and lower PTR. RBCs from EpoR-Cre GPX4f/f mice and control (GPX4f/f) mice were stored for 7 days under refrigerated conditions (n = 3), prior to PTR studies, which showed a significant drop in PTR in conditional GPX4 KO mice (A). After confirming GPX4 ablation through proteomics (B), we identified the top 50 metabolites (C) and proteins (D) affected by genotype and storage time (time series analysis of variance). Highlights include decreased consumption of free reduced glutathione (E), and increased accumulation of oxidized glutathione (F), increases in lipid hydroperoxides, and depletion of glutathionylated-adducts of lipid hydroperoxides (G-H).

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