Probe design and experimental procedure for hc-LRS. (A) Comparison of the probes used for hc-LRS in this study with those used for NGS. (B) Sequencing depth and coverage of the target regions. (C) The experimental process from genomic DNA to the final sequencing library involves the following steps: fragmentation, precapture PCR, hybridization capture, postcapture PCR, and adapter ligation. The figure illustrates the results of capillary electrophoresis for the fragmentation products, precapture PCR products, postcapture PCR products, and the final library, showing a trend of decreasing fragment length throughout the experiment. The red and orange bands indicate the fragments introduced by Tn5 transposase, the dark red/red and yellow/orange bands represent the primers for precapture amplification, the blue bands indicate the targeted regions, and the black bands represent the hairpin adapter. UTR, untranslated regions; WES, whole-exome sequencing; RFU, relative fluorescence unit.