Deletion of Il6 in MSCs enhances chemosensitivity of AML mice to Ara-C. (A) Schematic diagram of the experimental process in the mouse model. (B) Kaplan-Meier survival curves of AML mice. (C) AML burden in the BM and PB of AML mice. (D) Spleen size and weight in Il6^fl/fl and Il6_Prrx1^–/– AML mice. (E) Liver size and weight in Il6^fl/fl and Il6_Prrx1^–/– AML mice. (F) H&E staining was used to assess the infiltration of AML cells in the BM, splenic, and hepatic tissues (scale bar, 100 μm). (G) FCM was used to quantify the infiltration ratio of AML cells in the splenic and hepatic tissues (n = 5 mice per group). Data are presented as mean ± SD. Log-rank (Mantel-Cox) test was used for survival study comparisons (panel B). Differences were analyzed using ordinary 1-way ANOVA with Tukey multiple comparisons test (panels C-E,G). ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. IACS, IACS-010759 (OXPHOS inhibitor); Nor, normal healthy mice.