Transcriptomic profile of cHL by EBV/HIV status. (A) Principal component analysis (PCA), where each point is a sample, and color represents EBV/HIV status to highlight variation in RNA expression by EBV/HIV status. (B) Differentially enriched pathways from the Hallmark gene set database as determined by gene set variation analysis. Only significantly altered pathways (Padj < .1) are included. P values were adjusting using Benjamini-Hochberg. (C) Volcano plot of differentially expressed genes by EBV in HIV^– tumors. Points indicate genes enriched in EBV^–/HIV^– (left) and EBV⁺/HIV^– (right) tumors. (D) Volcano plot of differentially expressed genes by HIV in EBV⁺ tumors. Points indicate genes enriched in EBV⁺/HIV^– (left) and EBV⁺/HIV⁺ (right) tumors. Cut-offs for inclusion: |Log₂FC| > 1, Padj < .05. lncRNA and Y chromosome genes were excluded from volcano plots. PC1, principal component 1; PC2, principal component 2.