Figure 6.
In vitro traumatization promotes TG on the surface of healthy ECFCs. (A) TG in PNP in the presence of healthy ECFCs (biological replicate, N = 5; technical replicate, n = 3), cultured under untrau or trau conditions for 2 hours (i), with TG parameters: lag time (ii), ETP (iii), peak thrombin (iv), and time to peak thrombin (v). (B) TG in PNP incorporating cell culture supernatants from 2-hour untrau or trau ECFCs (biological replicate, N = 5; technical replicate, n = 3) over time (i), with derived parameters: lag time (ii), ETP (iii), peak thrombin (iv), and time to peak thrombin (v). (C) TG in PNP in the presence of healthy ECFCs (biological replicate, N = 5; technical replicate, n = 3), cultured under untrau or trau conditions for 24 hours (i), with derived parameters: lag time (ii), ETP (iii), peak thrombin (iv), and time to peak thrombin (v). (D) TG in PNP incorporating cell culture supernatants from 24-hour untrau or trau ECFCs (biological replicate, N = 5; technical replicate, n = 3) over time (i), with derived parameters: lag time (ii), ETP (iii), peak thrombin (iv), and time to peak thrombin (v). Data are presented as median with interquartile range. Statistical analysis was performed using the Wilcoxon test for paired comparisons in panels Aii-v,Bii-v,Cii-v,Dii-v; P value <.05 was considered statistically significant.

In vitro traumatization promotes TG on the surface of healthy ECFCs. (A) TG in PNP in the presence of healthy ECFCs (biological replicate, N = 5; technical replicate, n = 3), cultured under untrau or trau conditions for 2 hours (i), with TG parameters: lag time (ii), ETP (iii), peak thrombin (iv), and time to peak thrombin (v). (B) TG in PNP incorporating cell culture supernatants from 2-hour untrau or trau ECFCs (biological replicate, N = 5; technical replicate, n = 3) over time (i), with derived parameters: lag time (ii), ETP (iii), peak thrombin (iv), and time to peak thrombin (v). (C) TG in PNP in the presence of healthy ECFCs (biological replicate, N = 5; technical replicate, n = 3), cultured under untrau or trau conditions for 24 hours (i), with derived parameters: lag time (ii), ETP (iii), peak thrombin (iv), and time to peak thrombin (v). (D) TG in PNP incorporating cell culture supernatants from 24-hour untrau or trau ECFCs (biological replicate, N = 5; technical replicate, n = 3) over time (i), with derived parameters: lag time (ii), ETP (iii), peak thrombin (iv), and time to peak thrombin (v). Data are presented as median with interquartile range. Statistical analysis was performed using the Wilcoxon test for paired comparisons in panels Aii-v,Bii-v,Cii-v,Dii-v; P value <.05 was considered statistically significant.

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