In vitro traumatization accelerates tPA-induced CL on the surface of healthy ECFCs while markedly delaying it in their releasates. (A) Clot formation and lysis in PNP over time in the presence of healthy ECFCs (biological replicate, N = 5; technical replicate, n = 6), cultured under untrau or trau conditions for 2 hours (i), with associated CLT₅₀ (ii). (B) Clot formation and lysis in PNP incorporating cell culture supernatants from 2-hour untrau or trau ECFCs (biological replicate, N = 5; technical replicate, n = 3) over time (i), with derived CLT₅₀ (ii). (C) Clot formation and lysis in PNP over time in the presence of healthy ECFCs (biological replicate, N = 5; technical replicate, n = 6), cultured under untrau or trau conditions for 24 hours (i), with associated CLT₅₀ (ii). (D) Clot formation and lysis in PNP incorporating cell culture supernatants from 24-hour untrau or trau ECFCs (biological replicate, N = 5; technical replicate, n = 3) over time (i), with derived CLT₅₀ (ii). Data are presented as median with interquartile range. Statistical analysis was performed using the Wilcoxon test for paired comparisons in panels Aii,Bii,Cii,Dii, with P value <.05 considered statistically significant.