Figure 4.
In vitro traumatization remodels the hemostatically active surface of ECFCs. (A) Immunofluorescent staining of ECFCs (biological replicate, N = 5; technical replicate, n = 3), cultured under untrau or trau conditions for 2 hours, for surface TM, syndecan-1, TFPI, and TF (i), with the corresponding MFI values normalized to the MFI of the nuclear stain DAPI (ii). (B) Immunofluorescent staining of ECFCs (biological replicate, N = 5; technical replicate, n = 3), cultured under untrau or trau conditions for 24 hours, for surface TM, syndecan-1, TFPI, and TF (i), with the corresponding MFI values normalized to the MFI of the nuclear stain DAPI (ii). Data are presented as median with interquartile range. Statistical analysis was performed using the Wilcoxon test for paired comparison in panels Aii,Bii; with P value <.05 considered statistically significant. MFI, mean fluorescence intensity.

In vitro traumatization remodels the hemostatically active surface of ECFCs. (A) Immunofluorescent staining of ECFCs (biological replicate, N = 5; technical replicate, n = 3), cultured under untrau or trau conditions for 2 hours, for surface TM, syndecan-1, TFPI, and TF (i), with the corresponding MFI values normalized to the MFI of the nuclear stain DAPI (ii). (B) Immunofluorescent staining of ECFCs (biological replicate, N = 5; technical replicate, n = 3), cultured under untrau or trau conditions for 24 hours, for surface TM, syndecan-1, TFPI, and TF (i), with the corresponding MFI values normalized to the MFI of the nuclear stain DAPI (ii). Data are presented as median with interquartile range. Statistical analysis was performed using the Wilcoxon test for paired comparison in panels Aii,Bii; with P value <.05 considered statistically significant. MFI, mean fluorescence intensity.

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