Specific lectin signatures drive subgrouping of MDS platelets. (A) Principal component analysis (PCA) plot displaying clustering of platelets collected from patients with MDS based on their glycan profiles. Samples were grouped into Group A (teal) and Group B (green). Patients’ disease statuses are defined as intermediate/low/very low risk (“LR,” closed triangle) or high/very-high risk (“HR,” open triangle) based on Revised International Prognostic Scoring System (IPSS-R) score. (B) Distribution of IPSS-R scores for patients in Group A and Group B. (C) PCA plot displaying clustering of platelets collected from patients with MDS based on transfusions received within 4 weeks of sample collection. Patient 49 received a bone marrow transplant. (D-E) Graphs showing statistically significant (indicated by ∗) drivers of variance for each principal component, PC1 (D) and PC2 (E), among glycan profiles from MDS platelets. (F) Bar plots showing statistically significant changes in lectin binding between platelets collected from healthy individuals vs those with MDS. Binding of specific lectins is quantified based on their quantile normalized fluorescence values. Lectins are organized based on their selectivity for carbohydrate epitopes with example glycan substrates shown. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; and ∗∗∗∗P < .0001.