Molecular determinants of CIC in the TME system. (A) Cell proliferation determined by the CFSE assay; cells cultured with BMF only (left); cells cultured with BMF and TME stimuli CpG+IL-15 (right). The percentage of cell in proliferation are calculated as follows: percent proliferation = daughter/(daughter + parental) × 100%. (B) The correlation between CLL cell proliferation (as quantitated by CSFE) and cell internalization. R2 and P value for simple linear regression are indicated. Blue circles represent samples with proliferation <50%; purple circles represent samples with proliferation >50%. Solid line is best fit line, and dotted lines are the 95% confidence intervals. (C) Table listing known ligand-receptor interactions and/or soluble factors in the TME. The last column indicates the types of transduced BMF created with lentiviral transduction. (D) Effects of CM from transduced BMF on CIC over time. Each line with different symbols represents a different patient sample.
