CIC in FL and CIC responses to BTKis and ven. (A) CIC in FSCCL cell line. Each dot represents 1 experiment. Ten repeat experiments are shown. (B) CIC increased with ex vivo ibr exposure in FL primary samples (n = 9). Cells were treated ex vivo with 0.4 μM for 5 hours. Each dot represents a case. Data were analyzed with paired, parametric, 2-tailed t test. (C) CIC increased upon exposure to the covalent BTKi zanu in CLL (n = 5). (D) CIC increased upon exposure to the noncovalent BTKi pirto in CLL (n = 10). (E) CIC in ven- vs ibr-treated cells (n = 10). Cells were treated for 5 hours. (F) Percentage of changes based on data in panel C: percent change = (drug – DMSO)/DMSO × 100%. Data were analyzed with paired, parametric, 2-tailed t test. (G) Apoptosis rate of CLL cells inside vs outside of BMF. Cells were treated with ibr (0.4 μM), Acala (0.5 μM), ven (0.2 μM), or ibr+ven for 8 hours. FLICA-positive cells were counted using confocal imaging, and 5 fields per condition per experiment were analyzed. Apoptosis rates were calculated as follows: apoptotic CIC/total CIC × 100% or apoptotic outside cells/total outside cells × 100%. Error bars represent the standard deviation of 3 independent experiments.