Specific immune changes in patients with BC, patients with CLL, AML, and lymphoma-bearing mice. (A) Determination of CD14⁺ HLA-DR^low monocytes by flow cytometry in patients with BC when ND and in CR compared with matched HCs. Each symbol represents an individual patient. Significance determined by the ordinary 1-way ANOVA using the Šidák multiple comparison. (B) Staining for intracellular enzymes that correlate with inhibitory function IDO1, Arg1, and COX2 in CD14⁺/HLA-DR^low compared with respective in-patient control of DR^high myeloid cells of patients in CR. Each symbol represents an individual patient, significance determined by the paired t tests. (C) The count of CD14⁺/HLA-DR^high is blotted against the time point of the individual blood draw. Each symbol indicates a single patient. Red line indicates linear regression. (D) Relative and absolute CD4⁺ T-cell activation (HLA-DR) and exhaustion (PD1) in survivors of BC was measured by flow cytometry compared with HCs. Each symbol represents an individual patient, P values were determined by ordinary 1-way ANOVA using the Šidák multiple comparison. (E) The rate of activated (HLA-DR) CD4⁺ T-cells was blotted against the time point of the individual blood draw. Each symbol indicates a single patient. Red line indicates linear regression. (F) Indicated T-cell subsets were measured by flow cytometry from untreated CLL in watch-and-wait. Each symbol represents an individual patient. Two-group comparison was done using the ordinary 1-way ANOVA using the Šidák multiple comparison. (G) Indicated T-cell subset changes of respective disease entities including AML were normalized to the mean of their own HC group as 1 and allow for data comparison between the groups as indicated using the ANOVA group comparisons. Gray boxes indicate disease-specific significant alterations. (H) Scheme of the BL6 systemic BCL mouse model. (I) Indicated organs were analyzed for alterations in the indicated T-cell populations including respective markers after tumor injection compared with tumor-free mice by flow cytometry. Each symbol indicates values from an individual mouse, P values were determined by unpaired t tests. For all panels, ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. Arg1, arginase-1; BL6, black 6; COX2, cyclooxygenase 2; IDO-1, indoleamine 2,3-dioxygenase 1; mCD4, murine CD4; ns, not significant; PD1, programmed cell death protein 1; periph., peripheral; Tn, naive T cells; w&w, watch-and-wait.