Long-term αLy6G treatment delays high-density bone accrual in control and Dmp1Cre.Socs3f/f mice. (A-B) Bone volume as a percentage of total volume at low (red), mid (yellow), and high (blue) densities, in the femoral metaphysis (A) and diaphysis (B) in control and Dmp1Cre.Socs3f/f mice treated with IgG or αLy6G, as shown in Figure 4A. Shown above graphs are results of 2-way ANOVA for genotype and treatment, with significant differences of treatment shown above graphs, colored according to total bone mass (black), low (red), mid (yellow), and high (blue) densities. Data are mean + SEM (n = 7-11 mice per group). (C-H) Slice-by-slice analysis of low-density (C-D), mid-density (E-F), and high-density (G-H) bone in the femora moving from the distal end (left) to the diaphysis (right) in the same samples. Data are mean (solid line) ± SEM (dashed line), with P values (dashed black line) showing effect of treatment at each slice by 2-way ANOVA with Šidák post hoc test.
