Long-term αLy6G treatment reduced trabecular bone volume in control and Dmp1Cre.Socs3^f/f mice by increasing bone resorption. Representative femoral micro-CT images (A) and trabecular bone volume (B), trabecular number (C), trabecular separation (D), trabecular thickness (E), and metaphyseal cortical porosity (F) from control (Socs3^f/f) and Dmp1Cre.Socs3^f/f mice treated with IgG or αLy6G, as outlined in Figure 4A. Scale bar, 200 μm. (G-I) Histomorphometry of osteoid surface (G), osteoblast surface (H), and osteoclast surface (I) in tibial trabecular bone of control (Socs3^f/f) and Dmp1Cre.Socs3^f/f mice treated with IgG or αLy6G; histological images are shown in supplemental Figure 1. Data are mean ± SEM, with individual results for each animal shown; results from 2-way ANOVA shown below each graph, including the interaction between both factors (genotype and treatment); numbers above each graph are P values from Šidák post hoc tests for the comparisons shown.