Long-term αLy6G treatment leads to low trabecular bone mass in C57BL/6 mice. (A) Treatment scheme: 6-week-old mice were treated with αLy6G (1A8) or IgG 5 times per week; after the first week, αLy6G-treated mice were supplemented with anti-rat IgG2a (MAR18.5) 3 times per week, until 12 weeks of age, when tissues were collected 24 hours after the final injection. (B) Quantitation of CD11b⁺Gr1⁺ cells, as a percentage of all cells in PB in mice treated with IgG or αLy6G, showing neutrophil depletion in blood. (C-G) Micro-CT analysis of trabecular bone showing representative images (C), trabecular bone volume (D), trabecular number (E), trabecular separation (F), and trabecular thickness (G). Scale bar, 200 μm. Data are mean ± SEM with individual results for each animal shown; results of Student t test are shown above each graph. PB, peripheral blood.