αLy6G treatment for 2 weeks increased immature neutrophils in BM. (A-B) Schematic of FACS approach showing gating in IgG (A) and anti-Ly6G (αLy6G)–treated (B) marrow after treatment for 2 weeks, as shown in Figure 2A. (C-E) BM neutrophil populations as a percentage of BM cells in control (Dmp1Cre) and Dmp1Cre.Socs3^f/f mice treated with αLy6G or IgG for 2 weeks, as shown in Figure 2A; shown are preneutrophils (CD11b⁺Gr1⁺CXCR4^hic-kit^int) (C), immature neutrophils (CD11b⁺Gr1⁺CXCR4^loc-kit^loCXCR2⁻) (D), and mature neutrophils (CD11b⁺Gr1⁺CXCR4^loc-kit^loCXCR2⁺) (E). Data are mean ± SEM, with individual results for each animal shown; 2-way ANOVA results, including the interaction between both factors (genotype and treatment) are shown below each graph, with P values for αLy6G post hoc analyses shown above each pair of the same genotype. BM, bone marrow.