Functional profiling of SARS-CoV-2 WT spike antibody responses. (A-G) Box plots of IgG1, IgG3, IgM, IgA, FcR2A, FcR2B, FcR3A, or FcR3B titers in log10 MFI. Lines represent the minimum, lower quartile, median, upper quartile, and maximum. Statistical significance between groups was tested with a nonparametric Kruskal-Wallis multiple comparisons test. P values are as indicated. (H) Truncated violin plot of phagoscores from ADCP assay. The dashed lines represent quartiles, with a solid black line for median. Statistical significance between groups was tested with a nonparametric Kruskal-Wallis multiple comparisons test. P values are indicated as follows: not significant (ns), P = .1234; ∗P = .0332; ∗∗P = .0021; ∗∗∗P = .0002; ∗∗∗∗P < .0001. (I) Truncated violin plot of phagoscores from the ADNP assay. The dashed lines represent quartiles, with a solid black line denoting the median. Statistical significance between groups was tested with a nonparametric Kruskal-Wallis multiple comparisons test. P values are indicated as follows: ns, P = .1234; ∗P = .0332; ∗∗P = .0021; ∗∗∗P = .0002; ∗∗∗∗P < .0001. (J) Box plot of antibody-dependent complement deposition, shown as the MFI of FITC⁺ beads. Lines represent the minimum, lower quartile, median, upper quartile, and maximum. Statistical significance between groups was tested with a nonparametric Kruskal-Wallis multiple comparisons test. P values are as indicated. (K-L) Truncated violin plots of antibody-dependent NK-cell activation: percentage of CD107a⁺ NK cells or percentage of IFN-γ⁺ NK cells. Dashed lines represent quartiles, with a solid black line denoting the median. Statistical significance between groups was tested with a nonparametric Kruskal-Wallis multiple comparisons test. P values are indicated as follows: ns, P = .1234; ∗P = .0332; ∗∗P = .0021; ∗∗∗P = .0002; ∗∗∗∗P < .0001. FITC, fluorescein isothiocyanate; IFN-γ, interferon gamma; MFI, median fluorescence intensity.