Enitociclib synergized with MI-463 in KMT2A-r leukemia cells. (A) KMT2A-r cell lines cells were treated with varying doses of enitociclib, either alone in combination with MI-463 (IC₂₅ value), for 72 hours. Data represented as mean ± SEM from 3 biological repeats. (B) Fa-CI plots were derived by Chou-Talalay method using CompuSyn software, and overall CI values are given for each combination. (C) Western blot shows changes in HOXA9 protein levels in KOPN-8 and INL2 cells treated with enitociclib (IC₅₀ value), MI-463 (IC₅₀ value), or a combination of both the agents for 24 hours. (D) Densitometry analysis shows changes in HOXA9 protein levels from 2 to 3 independent biological experiments. For 2 group comparisons, a Student t test was used. A P value <.05 was considered statistically significant. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.