iMer inhibits stable aggregate formation under conditions of physiologic shear. (A) Human WB was pulled vertically (bottom to top) across horizontally oriented fibrillar collagen by vacuum syringe at a venous shear rate of 750 s−1. Plts were stained with anti-CD41 antibody (blue), then counterstained with anti–P-selectin antibody (green). (B) Surface area coverage was calculated by densitometry and circularity measurements and was significantly higher in vehicle-treated human platelets (green, n = 7), compared with iMer-treated platelets (red, n = 7 independent experiment samples; P < .05, Wilcoxon signed pairs rank test). (C) Quantitation of brightfield images of platelets showing a higher proportion of small aggregates (1-2 and 3-10 platelets per aggregate) after pretreatment with iMer than samples pretreated with vehicle control, which contained higher proportions of large aggregates (11-50, 50-100, and >100 platelets per aggregate). plts, platelets.
