iMer and GAS6 bind directly in coimmunoprecipitation. Brackets depict protein added and blots are labeled by detection antibody as anti-MERTK (MERTK detect) or anti-GAS6 (GAS6 detect) as indicated. (A) Glutathione S-transferase–tagged GAS6/iMer complexes were detected by immunoprecipitating with anti-human MERTK antibody and immunoblotting with an anti-human GAS6 antibody. (B) Respectively, GAS6/iMer complexes were also detected when immunoprecipitated with anti-human GAS6 antibody and immunoblotting with anti-human MERTK antibody. Also shown are the input protein and same protein immunoprecipitation/immunoblot controls. (C) His-tagged GAS6 (200 nM) or 200 nM each GAS6 and iMer were immunoprecipitated with anti-human GAS6 antibody. iMer coimmunoprecipitates with the GAS6 and is detected with anti-human MERTK antibody. Also shown are control immunoprecipitations with anti-human MERTK and input iMer. (D) Respectively, 200 nM iMer or 200 nM each iMer and GAS6 were immunoprecipitated with anti-human MERTK antibody. GAS6 coimmunoprecipitates with the iMer and is detected with anti-human GAS6 antibody. Also shown are the control immunoprecipitations with anti-human GAS6 and input GAS6. Images are representative of 3 independent assays. IP, immunoprecipitation.