PR of Cryo impairs shear-induced VWF elongation. (A) Representative western blot of VWF multimers in each hemostatic adjunct source as well as in freshly isolated PPP and in freshly thawed NPP. (B) Densitometry values from a given profile section (low-molecular-weight multimers [LMWM], 1-4 lowest bands; high-molecular-weight multimers [HMWM] + intermediate-molecular-weight multimers [IMWM], ≥5) divided by the sum of the total densitometry values from all sections, multiplied by 100. Data tabulated from N = 6 sets of unique hemostatic adjuncts. (C) VWF activity in each adjunct as detected by the REAADS assay; data are normalized to VWF:Ag content. (D) VWF collagen binding in each adjunct as detected by the Technozym CBA enzyme-linked immunosorbent assay; data are normalized to VWF:Ag content. (E) Ristocetin-induced VWF-mediated platelet aggregation in each adjunct; data are normalized to VWF:Ag content. (F) Representative western blot of VWF multimers in either intact or vortexed Cryo and PR-CryoFC. (G) HMWM + IMWM (top) and LMWM (bottom) frequencies in intact and vortexed Cryo and PR-CryoFC. Values of mean frequency at the base of each bar. (H) ADAMTS13 activity in intact and vortexed Cryo and PR-CryoFC; all data are at assay maximum, indicating unaltered ADAMTS13 function. Data in panels B-E, G, and H are visualized as mean (standard deviation). Statistical comparisons between HMWM + IMWM or LMWM frequencies in panel B were performed using 2-way ANOVA, with Dunnett multiple comparison to PPP. ∗∗∗∗P < .0001. Statistical comparisons in panels C through E were performed using 1-way ANOVA, with Dunnett multiple comparison with Cryo; ns, P > .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. Statistical comparisons between intact or vortexed adjuncts in panels G and H were performed using 2-way ANOVA. ∗P < .05. I, intact; NPP, normal pooled plasma; ns, not significant; V, vortexed.