Cryo and FibCon both fail to rescue in vitro–induced lytic coagulopathy under physiological flow conditions. (A) Representative phase images of clot formation in the injury site over the course of perfusion experiments in the microfluidic model of bleeding. (B) BTs, (C) closure frequency (the percentage of experiments that achieved complete closure), and (D) terminal clot surface area for rCryo:lyWB and rFibCon:lyWB at all 3 shear rates. In panel B, dotted line indicates assay cutoff at 1200 seconds and is representative of no occlusion. (E) Lag-phase duration, (F) growth rates, (G) clot lysis duration, and (H) number of total independently identified events extracted from kinetic analyses of clot formation in rCryo:lyWB and rFibCon:lyWB (see supplemental Figure 2 and the supplemental Methods for details). For panels B through H, N = 6 biological replicates per group and data are represented as mean (standard deviation), except panel C, which is a visualization of the frequency. rCryo:lyWB and rFibCon:lyWB were compared using an unpaired Student t test (Mann-Whitney U test).