Platelet-targeted LRRC8A deletion impairs injury-induced arterial thrombosis, without prolonging tail bleeding. (A-C) Thrombosis in the cremasteric arterioles of Lrrc8a^fl/fl (WT) and Pf4-Cre;Lrrc8a^fl/fl (cKO) mice after laser-induced injury as observed by real-time confocal intravital microscopy (red, DyLight 649 anti-CD42C) (A) with median time course (B) and quantification of the fluorescent reporter at 60, 120, and 180 seconds after injury (n = 37 for both WT and cKO) (C). (D) Representative traces of arterial blood flow through the carotid artery of WT (top) and cKO (bottom) mice after FeCl₃-induced injury. (E) Median time taken for the carotid artery of WT (n = 8) and cKO (n = 7) mice to occlude after FeCl₃-induced injury. (F-G) Total bleeding time after tail tip amputation of WT (n = 10) and cKO (n = 10) mice (F), with corresponding Hb concentrations of red blood cell lysates collected during the assay (G). Data in panel C are represented as mean only, whereas data in panels B,E are represented as median. Data in panels F,G are represented as mean ± SEM. Statistical significance was determined by the Mann-Whitney U test for panels C,E, and by unpaired t test for panels F-G. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. Hb, hemoglobin.