Figure 6.
Intracellular signaling by IFN-α in cells from Jak2V617F and Jak2V617F;Tyk2−/− mice. (A) Phosphorylation of STAT1 and STAT3 in response to IFN-α stimulation in BM (left) and spleen (right) cells from WT, Tyk2−/−, Jak2V617F, and Jak2V617F;Tyk2−/− mice. Cells from each genotype were treated with murine IFN-α (1000 U/mL) for 15 minutes and lysed. Western blotting was performed with the indicated antibodies. Three independent experiments were performed, and the representative result was shown here. (B) The expression level of phosphoprotein in each type of mice is shown as the relative ratio to that in the total amount of each STAT (n = 3 for each genotype group). A bar graph depicts the results from 3 replicate experiments. P values were calculated using the Tukey test after 1-way ANOVA; ∗P < .05; ∗∗P < .01. n.s., not significant; pSTAT, phospho-signal transducers and activators of transcription.

Intracellular signaling by IFN-α in cells from Jak2V617F and Jak2V617F;Tyk2−/− mice. (A) Phosphorylation of STAT1 and STAT3 in response to IFN-α stimulation in BM (left) and spleen (right) cells from WT, Tyk2−/−, Jak2V617F, and Jak2V617F;Tyk2−/− mice. Cells from each genotype were treated with murine IFN-α (1000 U/mL) for 15 minutes and lysed. Western blotting was performed with the indicated antibodies. Three independent experiments were performed, and the representative result was shown here. (B) The expression level of phosphoprotein in each type of mice is shown as the relative ratio to that in the total amount of each STAT (n = 3 for each genotype group). A bar graph depicts the results from 3 replicate experiments. P values were calculated using the Tukey test after 1-way ANOVA; ∗P < .05; ∗∗P < .01. n.s., not significant; pSTAT, phospho-signal transducers and activators of transcription.

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