Knockout of Clec12a in NUP98::NSD1+NRASG12D-immortalized cells. (A) Experimental outline showing the generation of a Clec12a deletion in the NUP98::NSD1+NRASG12D model. (B) Gene expression of Clec12a in NUP98::NSD1+NRASG12D-immortalized cells transduced with 3 different sgRNAs against Clec12a and Clec12a WT. Clec12a WT cells were transduced with CTL lacZ vector. (C) Clec12a protein expression in Clec12a WT and knockout NUP98::NSD1+NRASG12D cells analyzed by flow cytometry. Cells were gated on GFP⁺BFP⁺dTomato⁺. Clec12a WT cells were transduced with CTL lacZ vector. (D) Sanger sequencing shows the genomic deletion of Clec12a in NUP98::NSD1+NRASG12D-immortalized cells using sgRNA Clec12a sgT44. 5-FU, 5-fluoruracil; BFP, blue fluorescent protein. GFP, green fluorescent protein; WT, wild-type.