Mutational spectrum of CHIP. (A) Oncoplot of mutations in CHIP-associated genes detected in the PB of patients with MM at diagnosis. Each column represents 1 tumor sample and each row a gene. The heat map is color coded according to (maximum) VAF at which each gene was found mutated in the corresponding sample, as depicted in the color-scale bar at the bottom. In case the variant was found also in DNA from BM PCs, the gene reveals a black contour. For mutations whose MM origin could not be excluded due to unavailability of PC DNA, genes are highlighted with a diagonal bar. The number and the types of mutations (as specified in the legend) identified in each gene are plotted on the right of the heat map, preceded by the indication of the percentage of mutated patients. The bar graph above the heat map represents the number of variants per patient. (B) Dynamics of CHIP-associated mutations detected in longitudinally analyzed patients, suggesting patterns of branching (MM16) or linear evolution (MM4), or stability, expansion, or reduction of subclones present both at diagnosis and during follow-up (MM21, MM27, MM33, and MM9). The bar charts represent the VAF of the variants (listed in the legend on the right) at each time point (color coded according to the disease phase, as detailed under the graphs).