Gating of mono-blasts and distribution according to ELN risk categories. (A) CD45/SSC scatterplots of an illustrative case with FAB M4 AML, with backgating of expression of monocytic markers CD4 (brown), CD36 (red), and CD64 (purple). After exclusion of CD45^high/SSC^int mature monocytes (blue population), CD45^low/int/SSC^low-int gated AML blasts are divided into monoblasts expressing ≥2 of these monocytic markers (black population) and other blasts (green population). The proportion of monoblasts among total CD45⁺ hematopoietic cells (monoblasts/CD45⁺) is inspected as a biomarker of Ven-Aza. (B-C) Scatterplots of monoblasts/CD45⁺ according to ELN 2022 (B) and ELN 2024 risk categories (C). Bars represent mean value with standard deviation.