Enrichment of PD-1 blockade gene signature in CD4⁺ T cells in Pts receiving DVN therapy. GSEA analysis was performed on pooled CD4⁺ and CD8⁺ T-cell populations as defined by annotation of scRNA-seq data. Significantly enriched pathways were defined based on an adjusted P value <.05. For a complete list of significantly enriched pathways, see supplemental Tables 4-7. (A) Top 10 upregulated and downregulated GSEA pathways in baseline vs EOS CD4⁺ T-cell (left) and CD8⁺ T-cell (right) populations in Pts receiving DVN (n = 2). Top 10 pathways were determined based on NESs. Pathways upregulated in samples at baseline (blue) vs EOS (red) are shown with negative or positive NESs, respectively. (B) Upregulated GSEA pathways associated with PD-1 or TCR activity in CD4⁺ T cell at EOS compared with baseline. (C) GSEA plot showing upregulation of the WP_Cancer Immunotherapy by PD-1 Blockade and REACTOME_PD_1_SIGNALING pathways in CD4⁺ T cells at EOS compared with baseline. NES, normalized enrichment score.