Differential spectrum and effect of CREBBP mutations in human lymphoma. (A) Lollipop plots of the mutation spectrum of CREBBP from 1028 FL (left) and 3103 DLBCL (right) tumors. (B) The frequency of CREBBP hot spot mutations and nonsense/frameshift (truncating) mutations in FL (n = 1028), transformed FL (tFL) (n = 188), and DLBCL (n = 3103). (C) Western blot analysis of CREBBP and EP300 protein expression in CRISPR edited RL (left) and HT (right) lymphoma cell lines. (D-E) Unsupervised principal component analysis (PCA) of RNA-seq (D) and H3K27Ac CUT&RUN (E) data from CRISPR edited RL and HT lymphoma cell lines.