Biological characteristics and survival in TP53^mut MDS-EB1 and -EB2 with VAF ≥10%. (A) The median OS of patients with MDS-EB1, MDS-EB2, and AML with a VAF ≥10% was significantly poor compared with those with a VAF <10%. (B) Patients with MDS-EB1, MDS-EB2, or AML with a VAF <10% and a CK had significantly poorer median OS compared with those without CK. (C) Most MDS-EB1 and EB2 cases had VAF ≥10%. (D) The frequency of high-risk cytogenetic features, including CK, MK, 17p loss, deletion 5q, and deletion 7q, was similar between MDS-EB1 and MDS-EB2 cases with VAF ≥10%. (E) The median OS of patients with MDS-EB1 was comparable to that of patients with MDS-EB2. (F) For MDS-EB1 cases with VAF ≥10%, the median OS was similar between those with monoallelic and biallelic inactivation. (G) Similarly in MDS-EB2 cases with VAF ≥10%, the median OS was comparable between monoallelic and biallelic inactivation. 95% CI, 95% confidence interval; NA, not available.