CD41⁺ cells persist over the course of BMF with increased expression of IA-IE and CD53. (A) Representative flow cytometry plots of a gating strategy used to identify large cells with CD41⁺ expression. (B) Representative flow cytometry plots demonstrating CD41⁺, the large-cell population over the course of BMF, and individual results of absolute number of CD41⁺ large cells. (C) Changes of absolute numbers of CD11b during BMF. Representative flow cytometry plots showing IA-IE and CD53 in the CD41⁺ large-cell population over the course of BMF, and individual results of absolute number of IA-IE⁺CD41⁺ cells (D) and CD53⁺CD41⁺ cells (E). CON (n = 11), day 7 (n = 5), day 10 (n = 5), day 14 (n = 5), and day 17 (n = 5). (F) Frequencies and absolute numbers of the ploidy of MKs in normal mice and BMF mice. (G) Frequencies and absolute numbers of the ploidy of IA-IE⁺ MKs in normal mice and BMF mice. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. CON, control; D, day; FSC-A, forwad scatter area; NC, normal control; SSC-A, side scatter area.