DSS colitis susceptibility in mice with CGD is driven by the intestinal microbiota. DSS-treated WT, gp91^phox–/–, and p47^phox–/– mice from IRCM (n = 13-22) (A), the NIH (n = 15-25) (B), and gp91^phox–/– mice cross-fostered with p47^phox–/– mice from the NIH (CF p47^phox–/–; n = 3) (C-D) were evaluated for body weight, disease activity index, survival, colon length, and histology score. Representative distal colon sections stained with hematoxylin and eosin (original magnification ×20) are shown in panels A and B. All scale bars, 50 μm. (C) Principal coordinates analysis (PCoA) of β-diversity measured using Bray-Curtis dissimilarity for the comparison of gp91^phox–/–, p47^phox–/–, and gp91^phox–/– (CF p47^phox–/–) mice from the NIH at baseline. P values for the pairwise comparisons are shown in the table. All data are presented as means ± the standard error of the mean (SEM) from 2 to 4 independent experiments. Significance was determined using 1-way analysis of variance (ANOVA) and 2-way ANOVA with Holm-Šídák multiple comparison test. ∗P <. 05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.