Polyclonal cell selection and marker genes expression by clinical stages. (A) UMAP of scRNA-seq data of pPCs with colors indicating clinical clusters. Numerical values are reported for each category. (B) Dot plot displaying the top 20 marker genes that distinguish each clinical state. The x-axis lists the clinical category, whereas the y-axis lists gene names. Circle size corresponds to the number of cells in the category expressing the gene of interest, whereas the color shade correlates with the level of expression. (C) Violin plot showing expression pattern of selected PCs, adhesion/interaction, and autophagy marker genes across all the clinical stages.