Gene expression profiles of Abx-treated myeloma cells. (A) RNA sequence and GSEA by GO gene sets (C5) of Abx-treated vs untreated KMS34 and KMS21 cells. The RNA sequence data were used to elucidate the gene sets that were upregulated or downregulated (log₂FC > 0.1 or log₂FC less than −0.1) in both the Abx-treated cell lines and fulfilled P value of <.05 and false discovery rate of <0.25. The top 20 gene sets were enumerated by NES score. Among them, the enrichment plots for the 3 gene sets whose names included “autophagy” and “autophagosome” are also revealed. (B) To search for the targeting gene of Abx, heat maps of the genes included in the gene set “GOBP_NEGATIVE_REGULATION_OF_AUTOPHAGY” were enumerated. Expression levels of Sirt2 and FOXK1 were examined by western blots. All blots were repeated 3 times, and the signals were quantified by ImageJ scanning and adjusted with β-actin signals. ∗P < .05 vs 0 hour. (C) Pathway analysis of Abx-treated KMS34 and KMS21 cells was done using the genes with absolute log₂FC of >1 or log₂FC less than −1 (Ingenuity Pathway Analysis; Qiagen). GO, Gene Ontology; NES, Normalized Enrichment Score.