Figure 6.
The UPR induced by Abx treatment. (A) Expression of UPR molecules by Abx treatment of KMS34 myeloma cells over time. KMS34 cells were treated with 200 μM Abx for the indicated times. Expression levels of UPR molecules such as eIF2⍺, p-eIF2⍺, XBP1, XBP1s, and CHOP were evaluated by western blots. ∗P < .05 vs 0 hour. (B) Inhibition of UPR response and cell viability in Abx-treated KMS34 cells. KMS34 cells were treated with 2 μM GSK2606414 (a PERK inhibitor) or 60 μM STF083010 (an inositol-requiring enzyme 1⍺ inhibitor) for 1 hour. Then, 200 μM Abx was added to the culture medium, and the cells were incubated for an additional 6 hours. The viable cells were counted by trypan blue exclusion assay using an automatic cell counter. Expression levels of eIF2⍺, p-eIF2⍺, XBP1, XBP1s, and CHOP were evaluated by western blot analyses. ∗P < .05.

The UPR induced by Abx treatment. (A) Expression of UPR molecules by Abx treatment of KMS34 myeloma cells over time. KMS34 cells were treated with 200 μM Abx for the indicated times. Expression levels of UPR molecules such as eIF2⍺, p-eIF2⍺, XBP1, XBP1s, and CHOP were evaluated by western blots. ∗P < .05 vs 0 hour. (B) Inhibition of UPR response and cell viability in Abx-treated KMS34 cells. KMS34 cells were treated with 2 μM GSK2606414 (a PERK inhibitor) or 60 μM STF083010 (an inositol-requiring enzyme 1⍺ inhibitor) for 1 hour. Then, 200 μM Abx was added to the culture medium, and the cells were incubated for an additional 6 hours. The viable cells were counted by trypan blue exclusion assay using an automatic cell counter. Expression levels of eIF2⍺, p-eIF2⍺, XBP1, XBP1s, and CHOP were evaluated by western blot analyses. ∗P < .05.

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